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KMID : 0861020110260010041
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Korea Journal of Herbology
2011 Volume.26 No. 1 p.41 ~ p.46
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Simultaneous determination of baicalin and glycyrrhizin in Sosiho-tang by HPLC and anti-inflammatory activity
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Kim Jung-Hoon
Ha Hye-Kyung
Seo Chang-Seob
Lee Jin-Ah
Shin Hyeun-Kyoo
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Abstract
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Objectives: To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated.
Methods: A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin (PE)E₂ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats.
Results:The correlation coefficients of the compounds showed good linearity (r² > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on PGE₂ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats.
Conclusions :The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.
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KEYWORD
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Sosiho-tang (Xiao-Chai-Hu-Tang, SST), quantitative analysis, baicailn, glycyrrhizin, anti-inflammatory activity
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